Coding

Part:BBa_K187409

Designed by: David Lloyd   Group: iGEM09_Alberta   (2009-10-21)

trpA in pAB

trpA encodes the alpha subunit of tryptophan synthase, and is present in the standard biobrick vector pSB1A3.

This gene is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see RFC 47.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 823
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 823
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None